Reporter

Part:BBa_K2860003:Design

Designed by: Blacki Li Rudi Migliozzi   Group: iGEM18_Bio_Without_Borders   (2018-10-10)


GFP-cleavable linker-CBD-CBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 753
    Illegal AgeI site found at 1123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


Design Notes

Codon optimized for E. coli. Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix.


Source

This is an improvement on part Bba_K1321348. This part is composed of the ORF for Super-folder GFP fused N-terminally to dCBD (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.

References